Positive–negative-selection-mediated gene targeting in rice
نویسندگان
چکیده
منابع مشابه
Positive–negative-selection-mediated gene targeting in rice
Gene targeting (GT) refers to the designed modification of genomic sequence(s) through homologous recombination (HR). GT is a powerful tool both for the study of gene function and for molecular breeding. However, in transformation of higher plants, non-homologous end joining (NHEJ) occurs overwhelmingly in somatic cells, masking HR-mediated GT. Positive-negative selection (PNS) is an approach f...
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Gene targeting (GT) is a technique used to modify endogenous genes in target genomes precisely via homologous recombination (HR). Although GT plants are produced using genetic transformation techniques, if the difference between the endogenous and the modified gene is limited to point mutations, GT crops can be considered equivalent to non-genetically modified mutant crops generated by conventi...
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The modification of an endogenous gene into a designed sequence by homologous recombination, termed gene targeting, has broad implications for basic and applied research. Rice (Oryza sativa L.), with a sequenced genome of 389 Mb, is one of the most important crops and a model plant for cereals, and the single-copy gene Waxy on chromosome 6 has been modified with a frequency of 1% per surviving ...
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ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2015
ISSN: 1664-462X
DOI: 10.3389/fpls.2014.00748